DNA damage induced by etoposide; a comparison of two different methods for determination of strand breaks in DNA

Cancer Lett. 1996 Aug 2;105(2):153-9. doi: 10.1016/0304-3835(96)04266-8.

Abstract

Etoposide induces DNA damage to cells by interacting with the nuclear enzyme topoisomerase II. In this investigation the human lymphoblastic leukemia cell line (CEM) was used to study induction of DNA-strand breaks and cellular drug uptake after treatment with etoposide at a concentration of 0.5-2 micrograms/ml. High performance liquid chromatography was used for determination of etoposide concentrations. The alkaline elution assay and the DNA unwinding technique were compared for quantifying strand breaks in DNA induced by etoposide. The concentrations required to increase the level of DNA damage significantly was as follows: the DNA unwinding technique, 0.20 microgram/ml; the alkaline elution assay with proteinase K, 0.45 microgram/ml; the alkaline elution assay without proteinase K, 0.60 microgram/ml. When the half-life was adjusted, considering the efflux time of etoposide from cells, it was found to be only a few minutes. The present data show that the DNA unwinding technique is to be preferred for the screening of DNA damage. This technique is easier and quicker to perform than the alkaline elution technique.

Publication types

  • Comparative Study

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Chromatography, High Pressure Liquid
  • Confidence Intervals
  • DNA Damage*
  • DNA, Neoplasm / analysis
  • DNA, Neoplasm / drug effects*
  • DNA, Neoplasm / isolation & purification
  • DNA, Single-Stranded / chemistry
  • Etoposide / analysis
  • Etoposide / pharmacology*
  • Evaluation Studies as Topic
  • Genetic Techniques*
  • Humans
  • Leukemia, Lymphoid / drug therapy
  • Leukemia, Lymphoid / genetics
  • Regression Analysis
  • Time Factors
  • Tumor Cells, Cultured / chemistry

Substances

  • Antineoplastic Agents, Phytogenic
  • DNA, Neoplasm
  • DNA, Single-Stranded
  • Etoposide