Abstract
P.carinii molecular epidemiology appears a new interesting investigational field to understand distribution and incidence of isolates from different geographical locations. Recently a typing system, the Type Specific Oligoblotting (TSO) based on 6 different sequences of the Internal Transcribed Spacers (ITSs) of P.carinii rRNA has been developed [1]. By using P.carinii ITSs nested PCR followed by TSO hybridization we have typed 55 lung derived specimens collected in Italy, The Netherlands and sub-Saharian Africa from pts with microscopically detected P.carinii pneumonia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS-Related Opportunistic Infections / epidemiology
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AIDS-Related Opportunistic Infections / microbiology
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Adult
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Bronchoalveolar Lavage Fluid / microbiology
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DNA, Fungal / analysis
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Female
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Humans
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Italy / epidemiology
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Male
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Mycological Typing Techniques*
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Netherlands / epidemiology
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Pneumocystis / classification*
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Pneumocystis / genetics
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Pneumocystis / isolation & purification
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Pneumonia, Pneumocystis / epidemiology
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Pneumonia, Pneumocystis / microbiology
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Polymerase Chain Reaction / methods*
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Tanzania / epidemiology