Abstract
The mouse Htf9-a gene encodes a small hydrophilic protein, named Ran-binding protein 1 (RanBP-1), for its interaction with the Ras-related Ran protein; RanBP-1 binds the biologically active form of Ran. Ran has been implicated in a variety of nuclear events including DNA replication, RNA export and protein import, and monitoring completion of DNA synthesis before the onset of mitosis; it biological activity is thought to be regulated in S phase. We have investigated whether expression of the Htf9-a/RanBP-1 gene is linked to cell proliferation. Expression of the Htf9-a/RanBP-1 transcript appeared to be dependent on the proliferating state of the cells and peaked in S phase. We have identified a promoter region required for Htf9-a cell cycle-dependent expression and for responsiveness to cell cycle regulators. An E2F-binding site was identified within the cell cycle-regulated promoter region. Expression of the E1A oncoprotein prevented Htf9-a down-regulation in quiescent cells; in addition, both pRb and its relative p107 inhibited transcription from the Htf9-a promoter. These results link the control of Htf9-a/RanBP-1 expression to the cell cycle progression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Adenovirus E1A Proteins / biosynthesis
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Animals
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Base Sequence
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Binding Sites
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Carrier Proteins*
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Cell Cycle / physiology*
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Cell Cycle Proteins*
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Cell Division
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DNA-Binding Proteins*
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Down-Regulation
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E2F Transcription Factors
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GTP-Binding Proteins / genetics*
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Gene Expression Regulation / genetics*
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Liver / chemistry
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology
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Promoter Regions, Genetic / genetics
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Retinoblastoma Protein / physiology
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Retinoblastoma-Binding Protein 1
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Retinoblastoma-Like Protein p107
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Transcription Factor DP1
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Transcription Factors / metabolism
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Transcription, Genetic / genetics*
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ran GTP-Binding Protein*
Substances
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Adenovirus E1A Proteins
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Arid4a protein, mouse
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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E2F Transcription Factors
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Nuclear Proteins
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RNA, Messenger
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Rbl1 protein, mouse
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Recombinant Fusion Proteins
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Retinoblastoma Protein
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Retinoblastoma-Binding Protein 1
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Retinoblastoma-Like Protein p107
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Transcription Factor DP1
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Transcription Factors
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ran-binding protein 1
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GTP-Binding Proteins
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ran GTP-Binding Protein