Expression of jumper ant (Myrmecia pilosula) venom allergens: post-translational processing of allergen gene products

Biochem Mol Biol Int. 1996 Aug;39(5):877-85. doi: 10.1080/15216549600201022.

Abstract

N-terminal analyses of electrophoretically-separated allergenic polypeptides of the venom of the jumper ant M. pilosula showed that five out of the six allergenic polypeptides identified are homologous with the cloned major allergen Myr p I and may be derived from a single precursor polypeptide. The sixth polypeptide is homologous with a second cloned major allergen, Myr p II which is expressed as a single precursor polypeptide but exists in its native form as a disulphide bond-linked complex.

MeSH terms

  • Alkylation
  • Allergens*
  • Amino Acid Sequence
  • Animals
  • Ant Venoms / chemistry*
  • Ant Venoms / genetics*
  • Ant Venoms / immunology
  • Ants / genetics*
  • DNA, Complementary / immunology
  • Disulfides / chemistry
  • Electrophoresis, Polyacrylamide Gel / methods
  • Immunoblotting
  • Insect Proteins*
  • Molecular Sequence Data
  • Molecular Weight
  • Oxidation-Reduction
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • Protein Processing, Post-Translational*
  • Sequence Homology, Amino Acid

Substances

  • Allergens
  • Ant Venoms
  • DNA, Complementary
  • Disulfides
  • Insect Proteins
  • Myr p I
  • Myr p II protein, Myrmecia pilosula
  • Protein Precursors