Abstract
The Hs1(pro-1) locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet (Beta vulgaris L.). The Hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Membrane / chemistry
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Cloning, Molecular*
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DNA, Complementary / genetics
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Genes, Plant*
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Genetic Complementation Test
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Leucine / chemistry
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Membrane Proteins / physiology
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Molecular Sequence Data
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Nematoda / pathogenicity*
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Plant Diseases / genetics*
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Plant Diseases / parasitology
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Plant Proteins*
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Plant Roots / genetics
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Plant Roots / parasitology
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Transformation, Genetic
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Vegetables / genetics*
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Vegetables / parasitology*
Substances
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DNA, Complementary
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Hs1 pro-1 protein, Beta procumbens
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Membrane Proteins
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Plant Proteins
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Leucine