GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

Oncogene. 1997 Feb 27;14(8):997-1001. doi: 10.1038/sj.onc.1200898.

Abstract

Our laboratory previously described the independent isolation of the fibroblast growth factor 4 (FGF-4) gene by NIH3T3 transformation assay using DNA from a patient with CML leukemia (Lucas et al., 1994). The FGF-4 gene was truncated by DNA rearrangement with a novel gene named GRS. In this manuscript we describe isolation of GRS cDNA and show by sequence comparison that GRS is a novel member of the Bcl-2 gene family. Northern analysis shows expression of the gene in normal human tissue to be largely restricted to the hematopoietic compartment. Analysis of the pattern of gene expression in cancer cell lines demonstrates GRS is expressed in hematopoietic malignancies and in melanoma. The chromosomal location of GRS has also been determined. The gene is positioned on chromosome 15 within bands q24-25.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blood Cells / physiology
  • Chromosome Mapping
  • Chromosomes, Human, Pair 15
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors / genetics
  • Gene Expression
  • Genes, bcl-2*
  • Hematopoiesis
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukocytes / physiology*
  • Minor Histocompatibility Antigens
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Translocation, Genetic
  • Tumor Cells, Cultured

Substances

  • BCL2-related protein A1
  • FGF4 protein, human
  • Fibroblast Growth Factor 4
  • Minor Histocompatibility Antigens
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Fibroblast Growth Factors

Associated data

  • GENBANK/Y09397