Abstract
Nuclear-matrix proteins were prepared from different rat and human cells and separated by two-dimensional gel electrophoresis. By computer-assisted analysis of the images, two of the proteins were identified as ubiquitously occurring (common) nuclear-matrix proteins, which appeared in tissue-dependent concentrations. The two proteins that originated from human blood mononuclear cells were analyzed further. Tryptic digests of the blotted proteins were analyzed by partial peptide sequencing and matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry. The two human common nuclear-matrix proteins were identified as heterogeneous nuclear ribonucleoproteins (hnRNP) H and H' or their variants. Furthermore, mass analysis revealed details on the N terminus of hnRNP H.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Nuclear
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Electrophoresis, Gel, Two-Dimensional
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H
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Heterogeneous-Nuclear Ribonucleoproteins
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Humans
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Male
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Molecular Sequence Data
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Nuclear Matrix / chemistry*
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / isolation & purification*
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Rats
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Rats, Wistar
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Ribonucleoproteins / chemistry
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Ribonucleoproteins / genetics
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Ribonucleoproteins / isolation & purification*
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Sequence Homology, Amino Acid
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antigens, Nuclear
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H
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Heterogeneous-Nuclear Ribonucleoproteins
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Nuclear Proteins
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Ribonucleoproteins
Associated data
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GENBANK/L22009
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GENBANK/U01923