Abstract
The stimulation of immediate early gene expression in brain and neuronal cell culture systems has been reported after various experimental paradigms such as chemiconvulsant-provoked seizures or specific drug applications. In particular, the induction of immediate early genes by adrenergic model substances has been demonstrated by several investigators. This report demonstrates that a single dose of desipramine (10 or 25 mg/kg), a classical tricyclic antidepressant drug acting on the adrenergic system, induced c-fos and zif268 expression in rat hippocampus without affecting c-jun. The observed immediate early gene response might reflect part of a signal transduction cascade involved in long-term neuroadaptive and behavioral changes after antidepressant drug treatment.
MeSH terms
-
Animals
-
Antidepressive Agents, Tricyclic / pharmacology*
-
Brain Chemistry / drug effects*
-
DNA-Binding Proteins / biosynthesis
-
DNA-Binding Proteins / genetics
-
Desipramine / pharmacology*
-
Early Growth Response Protein 1
-
Gene Expression Regulation / drug effects*
-
Genes, Immediate-Early / drug effects*
-
Hippocampus / drug effects
-
Hippocampus / metabolism
-
Immediate-Early Proteins*
-
Locus Coeruleus / drug effects
-
Locus Coeruleus / metabolism
-
Male
-
Oligonucleotide Probes
-
Polymerase Chain Reaction
-
Proto-Oncogene Proteins c-fos / biosynthesis
-
Proto-Oncogene Proteins c-fos / genetics
-
Proto-Oncogene Proteins c-jun / biosynthesis
-
Proto-Oncogene Proteins c-jun / genetics
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Rats
-
Rats, Sprague-Dawley
-
Stimulation, Chemical
-
Transcription Factors / biosynthesis
-
Transcription Factors / genetics
Substances
-
Antidepressive Agents, Tricyclic
-
DNA-Binding Proteins
-
Early Growth Response Protein 1
-
Egr1 protein, rat
-
Immediate-Early Proteins
-
Oligonucleotide Probes
-
Proto-Oncogene Proteins c-fos
-
Proto-Oncogene Proteins c-jun
-
RNA, Messenger
-
Transcription Factors
-
Desipramine