Making chemistry selectable by linking it to infectivity

Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11777-82. doi: 10.1073/pnas.94.22.11777.

Abstract

The link between recognition and replication is fundamental to the operation of the immune system. In recent years, modeling this process in a format of phage-display combinatorial libraries has afforded a powerful tool for obtaining valuable antibodies. However, the ability to readily select and isolate rare catalysts would expand the scope of library technology. A technique in which phage infection controlled the link between recognition and replication was applied to show that chemistry is a selectable process. An antibody that operated by covalent catalysis to form an acyl intermediate restored phage infectivity and allowed selection from a library in which the catalyst constituted 1 in 10(5) members. Three different selection approaches were examined for their convenience and generality. Incorporating these protocols together with well known affinity labels and mechanism-based inactivators should allow the procurement of a wide range of novel catalytic antibodies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Catalytic / genetics*
  • Bacteriophages / pathogenicity
  • Base Sequence
  • Helper Viruses
  • Immunoglobulin Fragments / genetics
  • Immunoglobulin Variable Region / genetics
  • Molecular Biology / methods*
  • Molecular Sequence Data
  • Peptide Library*
  • Recombinant Fusion Proteins
  • Selection, Genetic*

Substances

  • Antibodies, Catalytic
  • Immunoglobulin Fragments
  • Immunoglobulin Variable Region
  • Peptide Library
  • Recombinant Fusion Proteins