Increase of foreign gene expression in monocot and dicot cells by an intron in the 5' untranslated region of a soybean phosphoenolpyruvate carboxylase gene

Biosci Biotechnol Biochem. 1998 Jan;62(1):151-3. doi: 10.1271/bbb.62.151.

Abstract

A genomic clone containing part of the coding region and upstream sequences of a phosphoenolpyruvate carboxylase (PEPC) gene was isolated from a soybean genomic library. The first intron of this gene is located in the 5' untranslated region. This intron-spanning fragment is capable of increasing GUS activity in plant cells.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • DNA, Plant
  • Gene Expression
  • Genes, Plant*
  • Genes, Reporter
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Glycine max / enzymology*
  • Glycine max / genetics
  • Introns*
  • Molecular Sequence Data
  • Phosphoenolpyruvate Carboxylase / biosynthesis
  • Phosphoenolpyruvate Carboxylase / genetics*
  • Protein Biosynthesis*

Substances

  • DNA, Plant
  • Glucuronidase
  • Phosphoenolpyruvate Carboxylase

Associated data

  • GENBANK/AB003908