Abstract
Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
4-Butyrolactone / analogs & derivatives*
-
4-Butyrolactone / metabolism
-
Bacterial Proteins / genetics
-
Conjugation, Genetic
-
DNA-Binding Proteins / genetics
-
Escherichia coli / genetics
-
Escherichia coli / growth & development
-
Escherichia coli / physiology*
-
Gene Expression Regulation, Bacterial*
-
Genes, Reporter
-
Genetic Vectors
-
Luminescent Measurements
-
Plasmids / genetics*
-
Repressor Proteins / genetics*
-
Signal Transduction / genetics*
-
Structure-Activity Relationship
-
Trans-Activators / genetics*
Substances
-
Bacterial Proteins
-
DNA-Binding Proteins
-
LasR protein, Pseudomonas aeruginosa
-
Repressor Proteins
-
RhlR protein, Pseudomonas aeruginosa
-
Trans-Activators
-
LuxR autoinducer binding proteins
-
homoserine lactone
-
4-Butyrolactone