Abstract
The glial cells missing (gcm) gene encodes an essential transcription factor that converts neuronal precursor cells to glial fate in the Drosophila nervous system. In this study, we tested effects of gcm ectopic expression on fate of non-neural cells. When gcm expression was continuously induced in epidermal cells from around stage 9, these cells started to exhibit mesenchymal cell morphology at stage 13, which was preceded by the onset of expression of Repo, a glial marker. The morphological change was coincident with loss of expression of an epidermal cell-adhesion molecule. In addition to the epidermis, fate of mesodermal cells was also affected by gcm ectopic expression. These findings suggest that gcm can convert gene expression and cell morphology even outside the neuroectoderm.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA-Binding Proteins
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Drosophila Proteins*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / embryology*
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Drosophila melanogaster / genetics*
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ELAV Proteins
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Ectoderm / cytology
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Ectoderm / metabolism
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Gene Expression Regulation, Developmental
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Genes, Insect*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Immunohistochemistry
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Insect Proteins / genetics
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Insect Proteins / metabolism
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Mesoderm / cytology
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Mesoderm / metabolism
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Neuroglia / cytology
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Neuroglia / metabolism
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Neuropeptides / genetics*
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Neuropeptides / metabolism
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Ribonucleoproteins / genetics
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Ribonucleoproteins / metabolism
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Trans-Activators / genetics*
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Trans-Activators / metabolism
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Transcription Factors
Substances
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DNA-Binding Proteins
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Drosophila Proteins
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ELAV Proteins
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GCM protein, Drosophila
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Homeodomain Proteins
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Insect Proteins
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Neuropeptides
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Ribonucleoproteins
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Trans-Activators
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Transcription Factors
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repo protein, Drosophila