Modulation of the neurotransmitter release in rat cerebellar neurons by GRO beta

Neuroreport. 1998 Nov 16;9(16):3601-6. doi: 10.1097/00001756-199811160-00011.

Abstract

We report here that, in cultured cerebellar granule cells, the CXC chemokine GRObeta stimulates the signaling pathway of the extracellular signal-regulated kinases, and enhances both evoked and spontaneous postsynaptic currents in patch clamped Purkinje neurons from rat cerebellar slices. The GRObeta-induced enhancement of the excitatory post-synaptic currents evoked by stimulating the parallel fibres is blocked by the inhibitor of the extracellular signal-regulated kinases pathway PD98059, which also reduces both basal frequency of spontaneous post-synaptic currents and mean amplitude of evoked excitatory post-synaptic currents. Our results suggest that GRObeta modulates neurotransmitter release in the cerebellum through the activation of the extracellular signal-regulated kinases pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / metabolism*
  • Enzyme Inhibitors / pharmacology
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • Flavonoids / pharmacology
  • Growth Inhibitors / metabolism*
  • Growth Substances / metabolism*
  • Intercellular Signaling Peptides and Proteins*
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases*
  • Neurotransmitter Agents / metabolism*
  • Organ Culture Techniques
  • Patch-Clamp Techniques
  • Purkinje Cells / cytology
  • Purkinje Cells / enzymology*
  • Rats
  • Signal Transduction / physiology
  • Synaptic Transmission / physiology
  • Tetrodotoxin / pharmacology

Substances

  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, rat
  • Enzyme Inhibitors
  • Flavonoids
  • Growth Inhibitors
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Neurotransmitter Agents
  • Tetrodotoxin
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one