Abstract
Actinomycin D, C2 and VII, cyclic peptides, inhibited Grb2 SH2 domain association (IC50 5-7 microM) with a phosphotyrosine containing peptide derived from the Shc protein (pTyr317). Actinomycins are the first examples of nonphosphorylated natural ligands of SH2 domain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Dactinomycin / analogs & derivatives*
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Dactinomycin / biosynthesis
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Dactinomycin / chemistry
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Dactinomycin / pharmacology*
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GRB2 Adaptor Protein
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Molecular Structure
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Proteins / antagonists & inhibitors*
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Ultraviolet
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Streptomyces / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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GRB2 Adaptor Protein
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Proteins
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actinomycin VII
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Dactinomycin
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actinomycin C2