Abstract
We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROalpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROalpha may play a neuromodulatory role on mouse cerebellum.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Animals, Newborn
-
Calcium / metabolism
-
Chemokine CXCL1
-
Chemokines, CXC*
-
Chemotactic Factors / pharmacology*
-
Cytosol / metabolism
-
Electric Conductivity
-
Excitatory Postsynaptic Potentials / drug effects
-
Excitatory Postsynaptic Potentials / physiology
-
Fluorescent Dyes
-
Growth Inhibitors / pharmacology*
-
Growth Substances / pharmacology*
-
Humans
-
In Vitro Techniques
-
Inositol 1,4,5-Trisphosphate / metabolism
-
Intercellular Signaling Peptides and Proteins*
-
Interleukin-8 / pharmacology*
-
Long-Term Potentiation / drug effects
-
Mice
-
Mice, Inbred BALB C
-
Organic Chemicals
-
Purkinje Cells / drug effects*
-
Purkinje Cells / metabolism
-
Purkinje Cells / physiology
-
Rats
-
Rats, Wistar
-
Receptors, Chemokine / metabolism
-
Receptors, Interleukin / metabolism
-
Receptors, Interleukin-8B
Substances
-
CXCL1 protein, human
-
Chemokine CXCL1
-
Chemokines, CXC
-
Chemotactic Factors
-
Cxcl1 protein, mouse
-
Cxcl1 protein, rat
-
Fluorescent Dyes
-
Growth Inhibitors
-
Growth Substances
-
Intercellular Signaling Peptides and Proteins
-
Interleukin-8
-
Organic Chemicals
-
Receptors, Chemokine
-
Receptors, Interleukin
-
Receptors, Interleukin-8B
-
calcium green
-
Inositol 1,4,5-Trisphosphate
-
Calcium