Abstract
Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acquired Immunodeficiency Syndrome / blood*
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Acquired Immunodeficiency Syndrome / virology
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Amino Acid Sequence
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Blood Specimen Collection / methods
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HIV Antibodies / blood
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HIV Antigens / chemistry
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HIV Antigens / immunology
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HIV-1 / classification*
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HIV-1 / isolation & purification*
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction / methods
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Reproducibility of Results
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Sensitivity and Specificity
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Serotyping / methods
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Thailand
Substances
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HIV Antibodies
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HIV Antigens