Samuelson JC - Search Results

1

Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants.

Samuelson JC, Morgan RD, Benner JS, Claus TE, Packard SL, Xu SY. Samuelson JC, et al. Nucleic Acids Res. 2006 Feb 2;34(3):796-805. doi: 10.1093/nar/gkj483. Print 2006. Nucleic Acids Res. 2006. PMID: 16456032 Free PMC article.

2

Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity.

Samuelson JC, Xu SY. Samuelson JC, et al. J Mol Biol. 2002 Jun 7;319(3):673-83. doi: 10.1016/S0022-2836(02)00343-1. J Mol Biol. 2002. PMID: 12054862

3

Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI.

Zhu Z, Samuelson JC, Zhou J, Dore A, Xu SY. Zhu Z, et al. Among authors: samuelson jc. J Mol Biol. 2004 Mar 26;337(3):573-83. doi: 10.1016/j.jmb.2004.02.003. J Mol Biol. 2004. PMID: 15019778

4

Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII.

Townson SA, Samuelson JC, Vanamee ES, Edwards TA, Escalante CR, Xu SY, Aggarwal AK. Townson SA, et al. Among authors: samuelson jc. J Mol Biol. 2004 May 7;338(4):725-33. doi: 10.1016/j.jmb.2004.02.074. J Mol Biol. 2004. PMID: 15099740

5

The isolation of strand-specific nicking endonucleases from a randomized SapI expression library.

Samuelson JC, Zhu Z, Xu SY. Samuelson JC, et al. Nucleic Acids Res. 2004 Jul 9;32(12):3661-71. doi: 10.1093/nar/gkh674. Print 2004. Nucleic Acids Res. 2004. PMID: 15247348 Free PMC article.

6

Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.

Townson SA, Samuelson JC, Xu SY, Aggarwal AK. Townson SA, et al. Among authors: samuelson jc. Structure. 2005 May;13(5):791-801. doi: 10.1016/j.str.2005.02.018. Structure. 2005. PMID: 15893669 Free article.

7

BstYI bound to noncognate DNA reveals a "hemispecific" complex: implications for DNA scanning.

Townson SA, Samuelson JC, Bao Y, Xu SY, Aggarwal AK. Townson SA, et al. Among authors: samuelson jc. Structure. 2007 Apr;15(4):449-59. doi: 10.1016/j.str.2007.03.002. Structure. 2007. PMID: 17437717 Free article.

8

Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI.

Xu SY, Zhu Z, Zhang P, Chan SH, Samuelson JC, Xiao J, Ingalls D, Wilson GG. Xu SY, et al. Among authors: samuelson jc. Nucleic Acids Res. 2007;35(14):4608-18. doi: 10.1093/nar/gkm481. Epub 2007 Jun 22. Nucleic Acids Res. 2007. PMID: 17586812 Free PMC article.

9

Structures of the rare-cutting restriction endonuclease NotI reveal a unique metal binding fold involved in DNA binding.

Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL. Lambert AR, et al. Structure. 2008 Apr;16(4):558-69. doi: 10.1016/j.str.2008.01.017. Structure. 2008. PMID: 18400177 Free PMC article.

10

Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA.

Zhang P, Too PH, Samuelson JC, Chan SH, Vincze T, Doucette S, Bäckström S, Potamousis KD, Schramm TM, Forrest D, Schwartz DC, Xu SY. Zhang P, et al. Among authors: samuelson jc. Protein Expr Purif. 2010 Feb;69(2):226-34. doi: 10.1016/j.pep.2009.09.003. Epub 2009 Sep 9. Protein Expr Purif. 2010. PMID: 19747545 Free PMC article.

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