Geiger R - Search Results

1

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.

Thielking V, Selent U, Köhler E, Wolfes H, Pieper U, Geiger R, Urbanke C, Winkler FK, Pingoud A. Thielking V, et al. Among authors: geiger r. Biochemistry. 1991 Jul 2;30(26):6416-22. doi: 10.1021/bi00240a011. Biochemistry. 1991. PMID: 1647200

2

Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.

Geiger R, Rüter T, Alves J, Fliess A, Wolfes H, Pingoud V, Urbanke C, Maass G, Pingoud A, Düsterhöft A, et al. Geiger R, et al. Biochemistry. 1989 Mar 21;28(6):2667-77. doi: 10.1021/bi00432a046. Biochemistry. 1989. PMID: 2499352

3

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.

Alves J, Rüter T, Geiger R, Fliess A, Maass G, Pingoud A. Alves J, et al. Among authors: geiger r. Biochemistry. 1989 Mar 21;28(6):2678-84. doi: 10.1021/bi00432a047. Biochemistry. 1989. PMID: 2659077

4

Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.

Oelgeschläger T, Geiger R, Rüter T, Alves J, Fliess A, Pingoud A. Oelgeschläger T, et al. Among authors: geiger r. Gene. 1990 Apr 30;89(1):19-27. doi: 10.1016/0378-1119(90)90201-2. Gene. 1990. PMID: 2142667

5

Restriction enzymes.

Pingoud A, Alves J, Geiger R. Pingoud A, et al. Among authors: geiger r. Methods Mol Biol. 1993;16:107-200. doi: 10.1385/0-89603-234-5:107. Methods Mol Biol. 1993. PMID: 19082972 No abstract available.

6

Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.

Wolfes H, Alves J, Fliess A, Geiger R, Pingoud A. Wolfes H, et al. Among authors: geiger r. Nucleic Acids Res. 1986 Nov 25;14(22):9063-80. doi: 10.1093/nar/14.22.9063. Nucleic Acids Res. 1986. PMID: 3024128 Free PMC article.

7

DNA cleavage by AatI and StuI is sensitive to Escherichia coli dcm methylation.

Song YH, Rueter T, Geiger R. Song YH, et al. Among authors: geiger r. Nucleic Acids Res. 1988 Mar 25;16(6):2718. doi: 10.1093/nar/16.6.2718. Nucleic Acids Res. 1988. PMID: 2834695 Free PMC article.

8

Plasmid instabilities of single and three-plasmid systems in Escherichia coli during continuous cultivation.

Maschke HE, Kumar PK, Geiger R, Schügerl K. Maschke HE, et al. Among authors: geiger r. J Biotechnol. 1992 Jul;24(3):235-51. doi: 10.1016/0168-1656(92)90034-7. J Biotechnol. 1992. PMID: 1368895

9

The application of a photon-counting camera in sensitive, bioluminescence-enhanced detection systems for nucleic acid hybridization. Ultrasensitive detection systems for protein blotting and DNA hybridization, III.

Hauber R, Geiger R. Hauber R, et al. Among authors: geiger r. J Clin Chem Clin Biochem. 1989 Jun;27(6):361-3. J Clin Chem Clin Biochem. 1989. PMID: 2754395

A relatively simple, bioluminescence-enhanced detection system for nucleic acid hybridization, using alkaline phosphatase as a label, was described recently (Hauber, R. & Geiger, R. (1988) Nucl. Acid Res. 16, 1213). The principle of detection is as follow …

A relatively simple, bioluminescence-enhanced detection system for nucleic acid hybridization, using alkaline phosphatase as a label, was de …

10

Synthesis and characterization of a bioluminogenic substrate for alpha-chymotrypsin.

Monsees T, Miska W, Geiger R. Monsees T, et al. Among authors: geiger r. Anal Biochem. 1994 Sep;221(2):329-34. doi: 10.1006/abio.1994.1421. Anal Biochem. 1994. PMID: 7810874

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