Pingoud A - Search Results

1

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.

Thielking V, Selent U, Köhler E, Wolfes H, Pieper U, Geiger R, Urbanke C, Winkler FK, Pingoud A. Thielking V, et al. Among authors: pingoud a. Biochemistry. 1991 Jul 2;30(26):6416-22. doi: 10.1021/bi00240a011. Biochemistry. 1991. PMID: 1647200

2

A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV.

Selent U, Rüter T, Köhler E, Liedtke M, Thielking V, Alves J, Oelgeschläger T, Wolfes H, Peters F, Pingoud A. Selent U, et al. Among authors: pingoud a. Biochemistry. 1992 May 26;31(20):4808-15. doi: 10.1021/bi00135a010. Biochemistry. 1992. PMID: 1591242

3

Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences.

Thielking V, Alves J, Fliess A, Maass G, Pingoud A. Thielking V, et al. Among authors: pingoud a. Biochemistry. 1990 May 15;29(19):4682-91. doi: 10.1021/bi00471a024. Biochemistry. 1990. PMID: 2372551

4

Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.

Geiger R, Rüter T, Alves J, Fliess A, Wolfes H, Pingoud V, Urbanke C, Maass G, Pingoud A, Düsterhöft A, et al. Geiger R, et al. Among authors: pingoud a, pingoud v. Biochemistry. 1989 Mar 21;28(6):2667-77. doi: 10.1021/bi00432a046. Biochemistry. 1989. PMID: 2499352

5

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.

Alves J, Rüter T, Geiger R, Fliess A, Maass G, Pingoud A. Alves J, et al. Among authors: pingoud a. Biochemistry. 1989 Mar 21;28(6):2678-84. doi: 10.1021/bi00432a047. Biochemistry. 1989. PMID: 2659077

6

Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases.

Wolfes H, Fliess A, Winkler F, Pingoud A. Wolfes H, et al. Among authors: pingoud a. Eur J Biochem. 1986 Sep 1;159(2):267-73. doi: 10.1111/j.1432-1033.1986.tb09863.x. Eur J Biochem. 1986. PMID: 3019685 Free article.

7

Spectroscopic and hydrodynamic studies reveal structural differences in normal and transforming H-ras gene products.

Pingoud A, Wehrmann M, Pieper U, Gast FU, Urbanke C, Alves J, Feuerstein J, Wittinghofer A. Pingoud A, et al. Biochemistry. 1988 Jun 28;27(13):4735-40. doi: 10.1021/bi00413a023. Biochemistry. 1988. PMID: 3048389

8

Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.

Fliess A, Wolfes H, Seela F, Pingoud A. Fliess A, et al. Among authors: pingoud a. Nucleic Acids Res. 1988 Dec 23;16(24):11781-93. doi: 10.1093/nar/16.24.11781. Nucleic Acids Res. 1988. PMID: 3062581 Free PMC article.

9

DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme.

Jeltsch A, Maschke H, Selent U, Wenz C, Köhler E, Connolly BA, Thorogood H, Pingoud A. Jeltsch A, et al. Among authors: pingoud a. Biochemistry. 1995 May 9;34(18):6239-46. doi: 10.1021/bi00018a028. Biochemistry. 1995. PMID: 7742329

10

Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.

Jeltsch A, Alves J, Wolfes H, Maass G, Pingoud A. Jeltsch A, et al. Among authors: pingoud a. Biochemistry. 1994 Aug 30;33(34):10215-9. doi: 10.1021/bi00200a001. Biochemistry. 1994. PMID: 8068662

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