Yasukawa K - Search Results

1

Two crystal structures of lysyl-tRNA synthetase from Bacillus stearothermophilus in complex with lysyladenylate-like compounds: insights into the irreversible formation of the enzyme-bound adenylate of L-lysine hydroxamate.

Sakurama H, Takita T, Mikami B, Itoh T, Yasukawa K, Inouye K. Sakurama H, et al. Among authors: yasukawa k. J Biochem. 2009 May;145(5):555-63. doi: 10.1093/jb/mvp014. Epub 2009 Jan 27. J Biochem. 2009. PMID: 19174549

2

Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization.

Inouye K, Minoda M, Takita T, Sakurama H, Hashida Y, Kusano M, Yasukawa K. Inouye K, et al. Among authors: yasukawa k. Protein Expr Purif. 2006 Apr;46(2):248-55. doi: 10.1016/j.pep.2005.07.023. Epub 2005 Aug 24. Protein Expr Purif. 2006. PMID: 16169746

3

Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin.

Yasukawa K, Kusano M, Nakamura K, Inouye K. Yasukawa K, et al. Protein Expr Purif. 2006 Apr;46(2):332-6. doi: 10.1016/j.pep.2005.08.021. Epub 2005 Sep 22. Protein Expr Purif. 2006. PMID: 16216528

4

Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin.

Kusano M, Yasukawa K, Hashida Y, Inouye K. Kusano M, et al. Among authors: yasukawa k. J Biochem. 2006 Jun;139(6):1017-23. doi: 10.1093/jb/mvj112. J Biochem. 2006. PMID: 16788052

5

Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity.

Tatsumi C, Hashida Y, Yasukawa K, Inouye K. Tatsumi C, et al. Among authors: yasukawa k. J Biochem. 2007 Jun;141(6):835-42. doi: 10.1093/jb/mvm087. Epub 2007 Apr 3. J Biochem. 2007. PMID: 17405799

6

A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli.

Yasukawa K, Kusano M, Inouye K. Yasukawa K, et al. Protein Eng Des Sel. 2007 Aug;20(8):375-83. doi: 10.1093/protein/gzm031. Epub 2007 Jul 6. Protein Eng Des Sel. 2007. PMID: 17616558

7

Purification to homogeneity of a neutral metalloproteinase from Streptomyces caespitosus.

Inouye K, Shimada T, Yasukawa K. Inouye K, et al. Among authors: yasukawa k. Biosci Biotechnol Biochem. 2007 Jul;71(7):1773-6. doi: 10.1271/bbb.70056. Epub 2007 Jul 7. Biosci Biotechnol Biochem. 2007. PMID: 17617707 Free article.

8

Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease.

Inouye K, Shimada T, Yasukawa K. Inouye K, et al. Among authors: yasukawa k. J Biochem. 2007 Sep;142(3):317-24. doi: 10.1093/jb/mvm134. Epub 2007 Jul 23. J Biochem. 2007. PMID: 17646180

9

Improvement in performance of affinity gels containing Gly-D-Phe as a ligand to thermolysin due to increasing the spacer chain length.

Inouye K, Nakamura K, Kusano M, Yasukawa K. Inouye K, et al. Among authors: yasukawa k. Biosci Biotechnol Biochem. 2007 Aug;71(8):2083-6. doi: 10.1271/bbb.70178. Epub 2007 Aug 7. Biosci Biotechnol Biochem. 2007. PMID: 17690447 Free article.

10

Improving the activity and stability of thermolysin by site-directed mutagenesis.

Yasukawa K, Inouye K. Yasukawa K, et al. Biochim Biophys Acta. 2007 Oct;1774(10):1281-8. doi: 10.1016/j.bbapap.2007.08.002. Epub 2007 Aug 14. Biochim Biophys Acta. 2007. PMID: 17869197

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