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Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC. Hewitt SN, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1006-9. doi: 10.1107/S1744309111022159. Epub 2011 Aug 13. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011. PMID: 21904041 Free PMC article.
Structure of a Nudix hydrolase (MutT) in the Mg(2+)-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever.
Buchko GW, Edwards TE, Abendroth J, Arakaki TL, Law L, Napuli AJ, Hewitt SN, Van Voorhis WC, Stewart LJ, Staker BL, Myler PJ. Buchko GW, et al. Among authors: hewitt sn. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1078-83. doi: 10.1107/S1744309111011559. Epub 2011 Aug 16. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011. PMID: 21904053 Free PMC article.
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.
Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Buchko GW, et al. Among authors: hewitt sn. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1129-36. doi: 10.1107/S1744309111006336. Epub 2011 Aug 16. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011. PMID: 21904062 Free PMC article.
Solution-state NMR structure and biophysical characterization of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis.
Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Buchko GW, et al. Among authors: hewitt sn. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1148-53. doi: 10.1107/S1744309111008189. Epub 2011 Aug 16. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011. PMID: 21904065 Free PMC article.
Probing conformational states of glutaryl-CoA dehydrogenase by fragment screening.
Begley DW, Davies DR, Hartley RC, Hewitt SN, Rychel AL, Myler PJ, Van Voorhis WC, Staker BL, Stewart LJ. Begley DW, et al. Among authors: hewitt sn. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1060-9. doi: 10.1107/S1744309111014436. Epub 2011 Aug 13. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011. PMID: 21904051 Free PMC article.
Structure of a CutA1 divalent-cation tolerance protein from Cryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis.
Buchko GW, Abendroth J, Clifton MC, Robinson H, Zhang Y, Hewitt SN, Staker BL, Edwards TE, Van Voorhis WC, Myler PJ. Buchko GW, et al. Among authors: hewitt sn. Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):522-30. doi: 10.1107/S2053230X14028210. Epub 2015 Apr 18. Acta Crystallogr F Struct Biol Commun. 2015. PMID: 25945704 Free PMC article.
23 results