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Structural basis for CRISPR RNA-guided DNA recognition by Cascade.
Jore MM, Lundgren M, van Duijn E, Bultema JB, Westra ER, Waghmare SP, Wiedenheft B, Pul U, Wurm R, Wagner R, Beijer MR, Barendregt A, Zhou K, Snijders AP, Dickman MJ, Doudna JA, Boekema EJ, Heck AJ, van der Oost J, Brouns SJ. Jore MM, et al. Among authors: brouns sj. Nat Struct Mol Biol. 2011 May;18(5):529-36. doi: 10.1038/nsmb.2019. Epub 2011 Apr 3. Nat Struct Mol Biol. 2011. PMID: 21460843
CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.
Westra ER, van Erp PB, Künne T, Wong SP, Staals RH, Seegers CL, Bollen S, Jore MM, Semenova E, Severinov K, de Vos WM, Dame RT, de Vries R, Brouns SJ, van der Oost J. Westra ER, et al. Among authors: brouns sj. Mol Cell. 2012 Jun 8;46(5):595-605. doi: 10.1016/j.molcel.2012.03.018. Epub 2012 Apr 19. Mol Cell. 2012. PMID: 22521689 Free PMC article.
CRISPR interference directs strand specific spacer acquisition.
Swarts DC, Mosterd C, van Passel MW, Brouns SJ. Swarts DC, et al. Among authors: brouns sj. PLoS One. 2012;7(4):e35888. doi: 10.1371/journal.pone.0035888. Epub 2012 Apr 27. PLoS One. 2012. PMID: 22558257 Free PMC article.
Degenerate target sites mediate rapid primed CRISPR adaptation.
Fineran PC, Gerritzen MJ, Suárez-Diez M, Künne T, Boekhorst J, van Hijum SA, Staals RH, Brouns SJ. Fineran PC, et al. Among authors: brouns sj. Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1629-38. doi: 10.1073/pnas.1400071111. Epub 2014 Apr 7. Proc Natl Acad Sci U S A. 2014. PMID: 24711427 Free PMC article.
107 results