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An efficient system for stable markerless integration of large biosynthetic gene clusters into Streptomyces chromosomes.
Csolleiova D, Knirschova R, Rezuchova B, Homerova D, Sevcikova B, Matulova M, Núñez LE, Novakova R, Feckova L, Javorova R, Cortés J, Kormanec J. Csolleiova D, et al. Among authors: homerova d. Appl Microbiol Biotechnol. 2021 Mar;105(5):2123-2137. doi: 10.1007/s00253-021-11161-w. Epub 2021 Feb 10. Appl Microbiol Biotechnol. 2021. PMID: 33564923
An efficient blue-white screening system for markerless deletions and stable integrations in Streptomyces chromosomes based on the blue pigment indigoidine biosynthetic gene bpsA.
Rezuchova B, Homerova D, Sevcikova B, Núñez LE, Novakova R, Feckova L, Skultety L, Cortés J, Kormanec J. Rezuchova B, et al. Among authors: homerova d. Appl Microbiol Biotechnol. 2018 Dec;102(23):10231-10244. doi: 10.1007/s00253-018-9393-7. Epub 2018 Sep 26. Appl Microbiol Biotechnol. 2018. PMID: 30259098
The linear plasmid pSA3239 is essential for the replication of the Streptomyces lavendulae subsp. lavendulae CCM 3239 chromosome.
Novakova R, Rückert C, Knirschova R, Feckova L, Busche T, Csolleiova D, Homerova D, Rezuchova B, Javorova R, Sevcikova B, Kalinowski J, Kormanec J. Novakova R, et al. Among authors: homerova d. Res Microbiol. 2021 Sep-Oct;172(6):103870. doi: 10.1016/j.resmic.2021.103870. Epub 2021 Sep 3. Res Microbiol. 2021. PMID: 34487842 Free article.
68 results