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Genome-scale CRISPR-Cas9 knockout screening in human cells.
Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Shalem O, et al. Science. 2014 Jan 3;343(6166):84-87. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Science. 2014. PMID: 24336571 Free PMC article.
Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Ran FA, et al. Nat Protoc. 2013 Nov;8(11):2281-2308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Nat Protoc. 2013. PMID: 24157548 Free PMC article.