Abstract
The "core" pentasaccharide region of high mannose asparagine-linked glycoproteins was synthesized using thioethyl donors and methyl triflate to promote coupling. All monosaccharide units were prepared from glycals. The central mannose residue was installed by inversion of the C2 hydroxyl of a glucosyl precursor. The protecting group scheme employed allows for the extension of the pentasaccharide through the terminal mannose units and a peptide can be coupled through transformation of the glycal.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Asparagine / chemistry
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Chromatography, Thin Layer
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Glycoproteins / chemistry*
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Magnetic Resonance Spectroscopy
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Mannose / chemistry*
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Mass Spectrometry
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Mesylates / chemistry*
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Optical Rotation
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Polysaccharides / chemical synthesis*
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Polysaccharides / chemistry
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Spectroscopy, Fourier Transform Infrared
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Sulfhydryl Compounds / chemistry*
Substances
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Glycoproteins
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Mesylates
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Polysaccharides
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Sulfhydryl Compounds
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methyl triflate
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Asparagine
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Mannose