Abundance of the Membrane Proteome in Yeast Cells Lacking Spc1, a Non-catalytic Subunit of the Signal Peptidase Complex

J Membr Biol. 2024 Aug;257(3-4):207-214. doi: 10.1007/s00232-024-00312-5. Epub 2024 Apr 17.

Abstract

The signal peptidase complex (SPC) mediates processing of signal peptides of secretory precursors. But, recent studies show that the eukaryotic SPC also cleaves internal transmembrane segments of some membrane proteins, and its non-catalytic subunit, Spc1/SPCS1 plays a critical role in this process. To assess the impact of Spc1 on membrane proteostasis, we carried out quantitative proteomics of yeast cells with and without Spc1. Our data show that the abundance of the membrane proteome in yeast cells lacking Spc1 is in general reduced compared to that in wild-type cells, implicating its role in controlling the cellular levels of membrane proteins.

Keywords: Membrane proteins; Quality control; Quantity control; SPCS1; Spc12.

MeSH terms

  • Aspartic Acid Endopeptidases / genetics
  • Aspartic Acid Endopeptidases / metabolism
  • Cell Membrane / metabolism
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Proteome* / metabolism
  • Proteomics / methods
  • Saccharomyces cerevisiae Proteins* / genetics
  • Saccharomyces cerevisiae Proteins* / metabolism
  • Saccharomyces cerevisiae* / genetics
  • Saccharomyces cerevisiae* / metabolism
  • Serine Endopeptidases

Substances

  • Proteome
  • Saccharomyces cerevisiae Proteins
  • Membrane Proteins
  • type I signal peptidase
  • Aspartic Acid Endopeptidases
  • Serine Endopeptidases