Abstract
Mitochondrial RNA turnover in yeast involves the degradosome, composed of DSS-1 exoribonuclease and SUV3 RNA helicase. Here, we describe a degradosome-like complex, containing SUV3 and DSS-1 homologues, in the early branching protozoan, Trypanosoma brucei. TbSUV3 is mitochondrially localized and co-sediments with TbDSS-1 on glycerol gradients. Co-immunoprecipitation demonstrates that TbSUV3 and TbDSS-1 associate in a stable complex, which differs from the yeast degradosome in that it is not stably associated with mitochondrial ribosomes. This is the first report of a mitochondrial degradosome-like complex outside of yeast. Our data indicate an early evolutionary origin for the mitochondrial SUV3/DSS-1 containing complex.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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Biological Evolution
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Endoribonucleases / metabolism*
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Exoribonucleases / genetics
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Exoribonucleases / metabolism
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Humans
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Mitochondria / genetics
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Mitochondria / metabolism*
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Molecular Sequence Data
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Multienzyme Complexes / metabolism*
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Polyribonucleotide Nucleotidyltransferase / metabolism*
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Protozoan Proteins / genetics
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Protozoan Proteins / metabolism*
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RNA Helicases / genetics
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RNA Helicases / metabolism*
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RNA, Protozoan
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Sequence Alignment
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Trypanosoma brucei brucei / cytology*
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Trypanosoma brucei brucei / metabolism
Substances
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Multienzyme Complexes
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Protozoan Proteins
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RNA, Protozoan
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degradosome
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Polyribonucleotide Nucleotidyltransferase
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Endoribonucleases
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Exoribonucleases
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RNA Helicases