Anopheles gambiae is the principal vector of malaria, a disease that afflicts more than 500
million people and causes more than 1 million deaths each year. Tenfold shotgun sequence …

We have applied “whole-genome shotgun sequencing” to microbial populations collected
en masse on tangential flow and impact filters from seawater samples collected from the …

The rhesus macaque (Macaca mulatta) is an abundant primate species that diverged from
the ancestors of Homo sapiens about 25 million years ago. Because they are genetically …

The freshwater cnidarian Hydra was first described in 1702 and has been the object of study
for 300 years. Experimental studies of Hydra between 1736 and 1744 culminated in the …

We have improved upon the methodology and dramatically shortened the time required for
accurate assembly of 5-to 6-kb segments of DNA from synthetic oligonucleotides. As a test of …

We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–
7 kb in size by> 109-fold, using φ29 DNA polymerase. The principal difficulty with …

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology
and serves as a model system for the investigation of many developmental and cellular …

We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma
mycoides JCVI-syn1. 0 genome starting from digitized genome sequence information and its …

The world's oceans contain a complex mixture of micro-organisms that are for the most part,
uncharacterized both genetically and biochemically. We report here a metagenomic study of …

We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic
genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium …