Fluid Thioglycollate Medium (Thioglycollate Medium W/ Indicator and Dextrose)

FLUID THIOGLYCOLLATE MEDIUM
(THIOGLYCOLLATE MEDIUM w/ INDICATOR and DEXTROSE)

INTENDED USE
Remel Fluid Thioglycollate Medium (Thioglycollate Medium w/ Indicator and Dextrose) is a general-purpose, liquid medium recommended for
use in qualitative procedures for the cultivation of aerobes and anaerobes and for sterility testing.
SUMMARY AND EXPLANATION

Thioglycollate Medium was first described by Brewer in 1940.1 This medium contained a small amount of agar and a reducing substance to
initiate the growth of anaerobes.2 Fluid Thioglycollate Medium is recommended by the United States Pharmacopeia (USP) and the AOAC
International (AOAC).3,4 It is also recommended by the American Public Health Association (APHA) and Food and Drug Administration (FDA) in
the Bacteriological Analytical Manual Online.5,6
PRINCIPLE
Sodium thioglycollate is a reducing agent which maintains a low oxygen tension in anaerobic media. A small amount of agar is added to
impede diffusion of oxygen. Casein peptone and cystine supply nitrogenous and carbon compounds, while the sodium chloride maintains
osmotic equilibrium. Resazurin is added as an oxidation-reduction indicator which turns pink when increased oxidation occurs. Yeast extract
supplies B-complex vitamins and enhances the growth of microorganisms.
REAGENTS (CLASSICAL FORMULA)*

Casein Peptone ............................................................. 15.0 g L-Cystine........................................................................ 0.5 g
Yeast Extract ................................................................... 5.0 g Sodium Thioglycollate .................................................... 0.5 g
Dextrose .......................................................................... 5.0 g Resazurin ....................................................................... 1.0 mg
Sodium Chloride .............................................................. 2.5 g Agar .............................................................................. 0.75 g
Demineralized Water ................................................1000.0 ml
pH 7.1 ± 0.2 @ 25°C
*Adjusted as required to meet performance standards.
PRECAUTIONS
This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions.
PREPARATION OF DEHYDRATED CULTURE MEDIUM

1. Suspend 29.5 g of medium in 1000 ml of demineralized water.
2. Heat to boiling with agitation to completely dissolve.
3. Dispense into appropriate containers and sterilize by autoclaving at 121°C for 15 minutes.
4. Store prepared media at 15-30°C.
5. If more than 30% of the medium is pink, reheat medium only once in a boiling water or steam bath (100°C) to drive off absorbed oxygen
prior to use.
PROCEDURE
1. Consult current editions of appropriate references for the recommended procedure for sample preparation, inoculation, testing, and
interpretation.
QUALITY CONTROL
Each lot number of Fluid Thioglycollate Medium has been manufactured, packaged, and processed in accordance with current Good
Manufacturing Practice regulations. All lot numbers have been tested using the following quality control organisms and have been found to
be acceptable. Testing of control organisms should be performed in accordance with established laboratory quality control procedures.
CONTROL INCUBATION RESULTS

Clostridium sporogenes ATCC® 11437 Ambient, up to 3 days @ 30-35°C Growth
Kocuria rhizophila ATCC® 9341 Ambient, up to 3 days @ 30-35°C Growth
Pseudomonas aeruginosa ATCC® 9027 Ambient, up to 3 days @ 30-35°C Growth
Staphylococcus aureus ATCC® 6538 Ambient, up to 3 days @ 30-35°C Growth
LIMITATIONS
1. A slight turbidity (haziness) may be present due to the small amount of agar in the medium.
2. Do not boil more than once; frequent boiling results in development of toxic products.
3. Slight to moderate color variation of broth media does not affect performance.
4. Nonviable organisms may be seen when gram staining some culture media resulting from their presence in various media components.
BIBLIOGRAPHY
1. Brewer, J.H. 1940. JAMA. 115:598-600.
2. MacFaddin, J.F. 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Williams & Wilkins, Baltimore, MD.
3. The United States Pharmacopeia. 2009. 32nd ed. United States Pharmacopeial Convention, Rockville, MD.
4. Horwitz, W. 2002. Official Methods of Analysis of AOAC International. 17th ed., 1st rev. AOAC International, Washington, D.C.
5. Food and Drug Administration. 2000. Bacteriological Analytical Manual Online. AOAC International, Gaithersburg, MD. http://www.fda.gov/Food/
ScienceResearch LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm055778.htm.
6. Downes, F.P. and K. Ito. 2001. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA, Washington, D.C.
Refer to the front of Remel Technical Manual of Microbiological Media for General Information regarding precautions, product storage and deterioration, sample
collection, storage and transportation, materials required, quality control, and limitations.
ATCC® is a registered trademark of American Type Culture Collection.

IFU 112640, Revised June 20, 2013 Printed in U.S.A.
12076 Santa Fe Drive, Lenexa, KS 66215, USA

General Information: (800) 255-6730 Website: www.remel.com Email: [email protected]
Local/International Phone: (913) 888-0939 International Fax: (913) 895-4128

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FLUID THIOGLYCOLLATE MEDIUM

(THIOGLYCOLLATE MEDIUM w/ INDICATOR and DEXTROSE)

SUMMARY AND EXPLANATION

REAGENTS (CLASSICAL FORMULA)*

PREPARATION OF DEHYDRATED CULTURE MEDIUM

CONTROL INCUBATION RESULTS

ATCC® is a registered trademark of American Type Culture Collection.

12076 Santa Fe Drive, Lenexa, KS 66215, USA

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