Zika Outbreak Toolbox

Suspected case:

A patient presenting with rash (usually maculopapular and pruritic)

and/or fever

and at least one of the following signs or symptoms:

Probable case:

Patient who meets the criteria of a suspected case, and has anti-ZIKV IgM antibodies, with negative laboratory results for other flaviviruses.

Confirmed case:

Patient who meets the criteria for a suspected case and has laboratory confirmation of recent ZIKV infection, with presence of:

ZIKV RNA or ZIKV antigen in serum samples or other specimens (e.g., urine, saliva, tissue, whole blood, or cerebrospinal fluid [CSF)] (Excluding abortion or stillbirth) or
positive anti-ZIKV IgM antibodies and plaque reduction neutralization test (PRNT) for ZIKV titers ≥ 10 in the absence of titers for other flaviviruses (The test is done on paired samples of probable cases with positive anti-ZIKV IgM antibodies).
In cases of death (Excluding abortion or stillbirth) molecular detection of the viral genome in autopsy tissue (fresh or in paraffin) with in situ hybridization tests.

Guidelines for surveillance of Zika virus disease and its complications (2018 edition) (Washington (DC): Pan American Health Organization, 2018 (Annex 2. Case definitions p. 38-42)).

Case investigation form(s):
- Zika complication: Guillain Barre - PDF | Word
- Zika complication: microcephalia - PDF | Word
Line list(s): Not available
Electronic investigation tools: Not available.