Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count

María J Ruiz-de-León; María A Jiménez-Sousa; Santiago Moreno; Marcial García; Mónica Gutiérrez-Rivas; Agathe León; Marta Montero-Alonso; Juan González-García; Salvador Resino; Norma Rallón; José M Benito; Alejandro Vallejo; ECRIS Network integrated in the Spanish AIDS Research Network

doi:10.1016/j.jmii.2018.07.007

Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count

J Microbiol Immunol Infect. 2019 Aug;52(4):667-671. doi: 10.1016/j.jmii.2018.07.007. Epub 2018 Aug 24.

Collaborators

ECRIS Network integrated in the Spanish AIDS Research Network:
J M Benito⁹, N Rallón⁹, C Restrepo⁹, N Rodríguez⁹, M García⁹, A Cabello⁹, M Gorgolas⁹, S Resino¹⁰, V Briz¹⁰, M A Jiménez¹⁰, M S Vázquez¹⁰, A Fernández¹⁰, P García¹⁰, M A Muñoz¹¹, J Sánchez¹¹, J L Jiménez¹¹, D Sepúlveda¹¹, I García¹¹, I Consuegra¹¹, A León¹², M Arnedo¹², M Plana¹², N Climent¹², F García¹², E Ruiz-Mateos¹³, B Domínguez¹³, L Tarancón¹³, M Rafii-El-Idrissi¹³, M J Polaino¹³, M Genebat¹³, P Viciana¹³, M Leal¹³, F Vidal¹⁴, E Rodríguez¹⁴, C Viladés¹⁴, J Peraire¹⁴, J Romero¹⁵, C Rodríguez¹⁵, M Vera¹⁵, J Esté¹⁶, E Ballana¹⁶, M A Martínez¹⁶, S Franco¹⁶, M Nevot¹⁶, A Vallejo¹⁷, S Moreno¹⁷, M Pernas¹⁰, C Casado¹⁰, C López¹⁰, L Capa¹⁰, M Pérez¹⁰, J Alcami¹⁰, R Sanjuán¹⁸, J M Cueva¹⁸, R Delgado¹⁹, O Sierra¹⁹, A Valenzuela²⁰

Affiliations

¹ Laboratory of Molecular Immunology, Department of Infectious Diseases, Instituto Ramón y Cajal Health Research Institute (IRyCIS), Ramón y Cajal University Hospital, Madrid, Spain.
² Viral Infection and Immunity Unit, National Center of Microbiology, Carlos III Health Institute, Majadahonda, Spain.
³ IIS-Jiménez Díaz Foundation, UAM, Madrid, Spain; Rey Juan Carlos University Hospital, Móstoles, Madrid, Spain.
⁴ Clinic Hospital-IDIBAPS, HIVACAT, Barcelona University, Barcelona, Spain.
⁵ Infectious Diseases Unit, La Fe University Hospital, Valencia, Spain.
⁶ Internal Medicine Department, La Paz University Hospital, Madrid, Spain.
⁷ IIS-Jiménez Díaz Foundation, UAM, Madrid, Spain; Rey Juan Carlos University Hospital, Móstoles, Madrid, Spain. Electronic address: [email protected].
⁸ Laboratory of Molecular Immunology, Department of Infectious Diseases, Instituto Ramón y Cajal Health Research Institute (IRyCIS), Ramón y Cajal University Hospital, Madrid, Spain. Electronic address: [email protected].
⁹ IS-Fundación Jimenez Díaz, UAM, Madrid, Spain.
¹⁰ Instituto Salud Carlos III, Madrid, Spain.
¹¹ Hospital Gregorio Marañón, Madrid, Spain.
¹² Hospital Clinic, Barcelona, Spain.
¹³ Hospital Virgen del Rocio, Sevilla, Spain.
¹⁴ Hospital Joan XXIII, Tarragona, Spain.
¹⁵ Centro Sandoval, Madrid, Spain.
¹⁶ Fundacion IRSI CAIXA, Badalona, Spain.
¹⁷ Hospital Ramón y Cajal, Madrid, Spain.
¹⁸ Universidad de Valencia, Spain.
¹⁹ Hospital 12 de Octubre, Madrid, Spain.
²⁰ Universidad de la Laguna, Sta. Cruz Tenerife, Spain.

PMID: 30193823
DOI: 10.1016/j.jmii.2018.07.007

Abstract

Exosome-derived miR-21 was independently associated with CD4 T cell decline in HIV-1-infected elite controllers (OR 0.369, 95% CI 0.137-0.994, p = 0.049). Also, a negative correlation between miR-21 expression and MCP-1 level was found (r = -0.649, p = 0.020), while no correlation between soluble biomarkers or cellular immune activation was found.

Keywords: Elite controllers; Exosomes; HIV-1; Immune activation; Soluble biomarkers; miRNAs.

MeSH terms

Adult
Biomarkers / blood
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes / immunology*
Chemokine CCL2 / blood
Cross-Sectional Studies
Exosomes*
Female
HIV Infections / immunology*
HIV-1
Humans
Male
MicroRNAs / blood*
Middle Aged
Plasma / immunology*

Substances

Biomarkers
CCL2 protein, human
Chemokine CCL2
MIRN21 microRNA, human
MicroRNAs