Genital infections in high-risk human papillomavirus positive Paraguayan women aged 30-64 with and without cervical lesions

PLoS One. 2024 Oct 29;19(10):e0312947. doi: 10.1371/journal.pone.0312947. eCollection 2024.

Abstract

Objective: To determine the prevalence of genital infections (GIs), including sexual transmitted STIs: Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Trichomonas vaginalis, and opportunistic pathogens that generally do not cause STIs, non-classic STI: Ureaplasma urealyticum, Ureaplasma parvum and Mycoplasma hominis, in women with high-risk oncogenic human papillomavirus (hr-HPV) infection and their association with cervical lesions.

Methods: A cross-sectional study was carried out including 231 hr-HPV positive women. Of these, 46 has histologically confirmed cervical intraepithelial neoplasia 3 (CIN3) or more (including CIN3 and cervical cancer lesions-CIN3+). GIs were detected by multiplex real time PCR. Odds ratios (OR) were estimated to explore possible associations between GIs and the presence or absence of CIN3+ lesions. Additionally, we examined associations between sociodemographic, sexual, and clinical characteristics and the presence of GIs.

Results: In total, there were 174/231 cases of GIs corresponding to an overall prevalence of 75.3% (95%CI: 69.4-80.4), being non-classic STIs the most common (72.3%) compared to STIs (12.6%). The most prevalent non-classic STI and STI were U. parvum (49.8%) and C. trachomatis (7.4%), respectively. The odds of presenting GIs were 3 times higher in women under 46 years compared to older counterparts (OR: 3.32, 95%CI: 1.74-6.16), and in women with a normal Pap smear with inflammation compared to those without inflammation (OR: 3.31, 95%CI: 1.15-9.77). GIs were equally present in women with and without CIN3+ lesions.

Conclusion: We observed an association of GIs with inflammation in the Pap smear, but no association with CIN3+, as some of them are very common and likely part of the normal vaginal flora, suggesting that such infections do not appear to be cofactors in cervical carcinogenesis, although larger prospective studies are needed.

MeSH terms

  • Adult
  • Cross-Sectional Studies
  • Female
  • Human Papillomavirus Viruses
  • Humans
  • Middle Aged
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections* / epidemiology
  • Papillomavirus Infections* / virology
  • Paraguay / epidemiology
  • Prevalence
  • Sexually Transmitted Diseases / epidemiology
  • Sexually Transmitted Diseases / microbiology
  • Sexually Transmitted Diseases / virology
  • Uterine Cervical Dysplasia / epidemiology
  • Uterine Cervical Dysplasia / microbiology
  • Uterine Cervical Dysplasia / virology
  • Uterine Cervical Neoplasms* / epidemiology
  • Uterine Cervical Neoplasms* / virology

Grants and funding

To carry out this component of the study, funding was received from the National Council of Science and Technology-CONACYT, within the project “Evaluation of molecular biomarkers for detection of the risk of persistent human papillomavirus-HPV infection and progression of cervical lesions in women initially HPV-positive with a diagnosis of CIN1 or lower. ESTAMPA – PY cohort study”. Code: CONACYT PINV 18-256. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.