Electrospun Biocomposite Polycaprolactone/Collagen Tubes as Scaffolds for Neural Stem Cell Differentiation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nanofiber Fabrication and Characterization
2.1.1. SEM Analysis
Fiber diameter (nm) | Adhesion (%) | Proliferation (%) | Differentiation (% neurons) |
330 ± 17 | 92 | 84 | 38 |
510 ± 21 | 83 | 90 | 80 |
640 ± 83 | 68 | 81 | 58 |
Fiber alignment | Adhesion (%) | Proliferation (%) | Differentiation (%) |
Random | 73 | 52 | 56 |
Aligned | 94 | 71 | 73 |
2.1.2. Tensile Strength
2.1.3. Degradation
2.2. Growth Factor Release
2.3. In vitro Differentiation and Immunocytochemisty
3. Experimental Section
3.1. Electrospinning of Nanofibers
3.2. Morphology and Characterization of Electrospun Nanofibers
3.3. Growth Factor Release
3.4. Tissue Preparation and Neural Stem Cell Cultures
3.5. In vitro Differentiation and Immunocytochemisty
3.6. Antibodies
3.7. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Hackett, J.M.; Dang, T.T.; Tsai, E.C.; Cao, X. Electrospun Biocomposite Polycaprolactone/Collagen Tubes as Scaffolds for Neural Stem Cell Differentiation. Materials 2010, 3, 3714-3728. https://doi.org/10.3390/ma3063714
Hackett JM, Dang TT, Tsai EC, Cao X. Electrospun Biocomposite Polycaprolactone/Collagen Tubes as Scaffolds for Neural Stem Cell Differentiation. Materials. 2010; 3(6):3714-3728. https://doi.org/10.3390/ma3063714
Chicago/Turabian StyleHackett, Joanne M., ThucNhi T. Dang, Eve C. Tsai, and Xudong Cao. 2010. "Electrospun Biocomposite Polycaprolactone/Collagen Tubes as Scaffolds for Neural Stem Cell Differentiation" Materials 3, no. 6: 3714-3728. https://doi.org/10.3390/ma3063714