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  • F5 Peka 3 Growth of Bacteria

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    The document describes an experiment to investigate why mango pickles last longer than fresh mango slices. It provides guidelines for writing a lab report, including sections for the problem statement, aim, hypothesis, variables, materials, procedure, observations, data analysis, conclusions, and answers to questions about inferences, relationships between variables, the definition of microbial growth, why pickles last longer, improving accuracy, validity of data, and an overall conclusion. The purpose is to understand the effect of pH on bacterial growth through a controlled experiment.

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    F5 PEKA 3 GROWTH OF BACTERIA

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    The document describes an experiment to investigate why mango pickles last longer than fresh mango slices. It provides guidelines for writing a lab report, including sections for the problem statement, aim, hypothesis, variables, materials, procedure, observations, data analysis, conclusions, and answers to questions about inferences, relationships between variables, the definition of microbial growth, why pickles last longer, improving accuracy, validity of data, and an overall conclusion. The purpose is to understand the effect of pH on bacterial growth through a controlled experiment.

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    © All Rights Reserved
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    1K views6 pages

    F5 Peka 3 Growth of Bacteria

    Uploaded by

    farah6214
    The document describes an experiment to investigate why mango pickles last longer than fresh mango slices. It provides guidelines for writing a lab report, including sections for the problem statement, aim, hypothesis, variables, materials, procedure, observations, data analysis, conclusions, and answers to questions about inferences, relationships between variables, the definition of microbial growth, why pickles last longer, improving accuracy, validity of data, and an overall conclusion. The purpose is to understand the effect of pH on bacterial growth through a controlled experiment.

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    of 6

    SEKOLAH SULTAN ALAM SHAH

    SCIENCE 1511

    PEKA 2

    Arina bought mango pickles and fresh mango slices from a fruit
    stall. On the next day, she found that the fresh mango slices were
    rotten but the mango pickles were still in good condition.

    Based on the statement, plan and run an experiment to study the above situation. You are
    asked to write a report for the experiment. Your report should contain:
    (a) Problem statement
    (b) Aim
    (c) Hypothesis
    (d) Variables
    (e) Apparatus and materials
    (f) Procedure
    (g) Observations
    (h) Interpret data
    (i) Draw conclusions

    Also should answer the following questions:


    1. State the inference of the observation in this experiment.

    2. State the relationship between the manipulated variable and the responding
    variable.

    3. Based on this experiment, state the operational definition for the growth of
    microorganisms.

    4. Why do mango pickles last longer?

    5. Based on your findings of this experiment, suggest improvements to obtain more


    accurate data.

    6. Is the data you obtained valid? Give your reasons.

    7. State the conclusion for this experiment.

    As you conduct the experiment, your manipulative skills and the practice of scientific
    attitudes and values will be assessed.
    PEKA 1

    Name :

    Form :

    Date :

    Theme :
    1 Maintenance and continuity of life

    Learning area :
    1.1 Microorganisms

    Content standard :
    1.2 World of microorganisms

    Learning standard :
    1.1.2 Conduct an experiment to show the presence of microorganisms

    Problem statement :
    Why do mango pickles last longer than fresh mangoes slices? K1P1

    Aim :
    To study the effect of pH on the growth of Bacillus sp. K1P2

    Hypothesis : K1P3
    Growth of Bacillus sp. is best at pH7

    Variables: K1P4a
    Manipulated : pH value

    Responding : Number of colonies of Bacillus sp. K1P4b

    Constant : Surrounding temperature


    K1P4c

    Materials :
    Bacillus sp. culture solution, sterile nutrient agar, dilute hydrochloric acid solution, dilute
    sodium hydroxide solution, distilled water.
    K1P5
    Apparatus :
    Three sterile Petri dishes with lids labeled A, B and C, and sterile measuring cylinder (10
    cm3), cellophane tape, label paper, sterile petri dish with lid, three beakers, three coils of wire,
    a Bunsen burner and three syringe rods.
    Procedure :

    1. A petri dish containing 10 cm3 of sterile agar is prepared.

    2. 1 cm3 of distilled water is spread over the surface of the sterile agar.

    3. The coil of wire is sterilized using a Bunsen burner flame until the coil burns.

    4. The cooled coil of wire was dipped into a culture solution of Bacillus sp.

    5. Bacillus sp. culture solution is spread over each surface of the nutrient agar in a zig-
    zag shape using a coil of wire.

    6. The petri dish is closed and the sides are wrapped with cellophane tape.
    K1P6
    7. Repeat Step 1 to step 6 by substituting the distilled water to dilute hydrochloric acid
    and dilute sodium hydroxide solution as shown below :

    K1P7

    8. The three petri dishes were inverted and stored in a covered cupboard for three days.

    9. The number of bacterial colonies in each petri dish was calculated and recorded in a
    table.

    Observation :
    Number of bacterial K3P1
    Petri dish
    colonies
    A = sterile nutrient agar + Bacillus sp. culture
    16
    + distilled water
    B = sterile nutrient agar + Bacillus sp. culture
    8
    + dilute hydrochloric acid solution
    C = sterile nutrient agar + Bacillus sp. culture
    4 K3P2
    + dilute sodium hydroxide solution
    K4P1a

    K4P1b

    K4P1c

    K4P1d

    1. State the inference of the observation in this experiment. K4P2a


    The number of bacterial colonies in distilled water is the most because distilled
    water is a neutral medium.

    2. State the relationship between the manipulated variable and the responding
    variable. K4P2b
    The highest bacterial growth is in streaked with unwashed fingers .

    3. Based on this experiment, state the operational definition for the growth of
    microorganisms.
    Bacterial growth is a process that produces a number of colonies
    K4P2c

    4. Why do mango pickles last longer?


    Mango pickles are soaked in a solution of vinegar and salt. Salt will hydrate mango
    while vinegar is an acidic solution that will prevent bacteria from growing. K5P1

    5. Based on your findings of this experiment, suggest improvements to obtain more


    accurate data. K5P2
    This experiment should be carried out 3 times for a better results.
    6. Is the data you obtained valid? Give your reasons.
    Yes because bacterial colonies can be counted clearly. Also, the nutrient agar and K5P3
    Petri dishes were sterilized to kill bacteria. Furthermore, Petri dishes were cover
    with lids and sealed with cellophane tape to trap bacteria in Petri dishes. Petri
    dishes were inverted to prevent condensed water from falling onto the agar surface
    which will affect the growth of bacteria on the agar surface. K5P4

    7. State the conclusion for this experiment. K4P3


    Bacterial growth is best suited at pH7.
    Acidic or alkaline solution will inhibit the growth of bacteria.
    TUGASAN 1

    Arina membeli jeruk mangga serta potongan buah mangga segar yang dijual di sebuah
    gerai. Keesokan harinya, dia mendapati potongan buah mangga segar telah rosak manakala
    jeruk mangga masih dalam keadaan baik.

    Berdasarkan pernyataan di atas, rancang dan jalankan eksperimen untuk mengkaji situasi di
    atas .

    Di akhir eksperimen, anda perlu menulis satu laporan lengkap dan mengolah data dalam set
    komunikasi yang sesuai dan menjawab soalan berikut :

    1. Nyatakan inferens bagi pemerhatian dalam eksperimen ini.


    2. Nyatakan hubungan antara pemboleh ubah dimanipulasikan dengan pemboleh ubah
    bergerak balas.
    3. Berdasarkan eksperimen ini, nyatakan definisi secara operasi bagi pertumbuhan
    mikroorganisma.
    4. Mengapakah jeruk mangga tahan lebih lama?
    5. Berdasarkan dapatan eksperimen ini, cadangkan penambahbaikan untuk
    mendapatkan data yang lebih jitu.
    6. Adakah data yang anda perolehi sah? Berikan alasan anda.
    7. Nyatakan kesimpulan bagi eksperimen ini.

    Semasa anda menjalankan eksperimen, kemahiran manipulatif dan amalan sikap saintifik dan
    nilai murni akan dinilai.

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