Epidemics and pandemics of influenza are characterized by rapid global spread mediated by non-mutually exclusive transmission modes. The relative significance between contact, droplet, and airborne transmission is yet to be defined, a knowledge gap for implementing evidence-based infection control measures. We devised a transmission chamber that separates virus-laden particles by size and determined the particle sizes mediating transmission of influenza among ferrets through the air. Ferret-to-ferret transmission was mediated by airborne particles larger than 1.5 mu m, consistent with the quantity and size of virus-laden particles released by the donors. Onward transmission by donors was most efficient before fever onset and may continue for 5 days after inoculation. Multiple virus gene segments enhanced the transmissibility of a swine influenza virus among ferrets by increasing the release of virus-laden particles into the air. We provide direct experimental evidence of influenza transmission via droplets and fine droplet nuclei, albeit at different efficiency.

Jie Zhou; Jianjian Wei; Ka-Tim Choy; Sin Fun Sia; Dewi K. Rowlands; Dan Yu; Chung-Yi Wu; William G. Lindsley; Benjamin J. Cowling; James McDevitt; Malik Peiris; Yuguo Li; Hui-Ling Yen. Defining the sizes of airborne particles that mediate influenza transmission in ferrets. 2018, 115, E2386 -E2392.

Jie Zhou, Jianjian Wei, Ka-Tim Choy, Sin Fun Sia, Dewi K. Rowlands, Dan Yu, Chung-Yi Wu, William G. Lindsley, Benjamin J. Cowling, James McDevitt, Malik Peiris, Yuguo Li, Hui-Ling Yen. Defining the sizes of airborne particles that mediate influenza transmission in ferrets. . 2018; 115 (10):E2386-E2392.

Jie Zhou; Jianjian Wei; Ka-Tim Choy; Sin Fun Sia; Dewi K. Rowlands; Dan Yu; Chung-Yi Wu; William G. Lindsley; Benjamin J. Cowling; James McDevitt; Malik Peiris; Yuguo Li; Hui-Ling Yen. 2018. "Defining the sizes of airborne particles that mediate influenza transmission in ferrets." 115, no. 10: E2386-E2392.

Ping‐Ping Sun; Xiao‐Juan Yu; Su‐Xia Wang; Xu‐Jie Zhou; Lei Qu; Fan Zhang; Yi‐Yi Ma; Gang Liu; Li Yang. Association of vascular endothelial growth factor and renal thrombotic microangiopathy‐like lesions in patients with Castleman's disease. Nephrology 2019, 25, 125 -134.

Ping‐Ping Sun, Xiao‐Juan Yu, Su‐Xia Wang, Xu‐Jie Zhou, Lei Qu, Fan Zhang, Yi‐Yi Ma, Gang Liu, Li Yang. Association of vascular endothelial growth factor and renal thrombotic microangiopathy‐like lesions in patients with Castleman's disease. Nephrology. 2019; 25 (2):125-134.

Ping‐Ping Sun; Xiao‐Juan Yu; Su‐Xia Wang; Xu‐Jie Zhou; Lei Qu; Fan Zhang; Yi‐Yi Ma; Gang Liu; Li Yang. 2019. "Association of vascular endothelial growth factor and renal thrombotic microangiopathy‐like lesions in patients with Castleman's disease." Nephrology 25, no. 2: 125-134.

Background: The androgen receptor (AR) pathway drives most metastatic castration-resistant prostate cancers (mCRPC) even in late stages of the disease and resistance to ligand binding domain (LBD)-linked therapies inevitably emerges. Mechanisms of resistance to anti-androgens include AR gene amplification, C-terminal ligand-binding domain (LBD) mutations and expression of constitutively-active truncated AR splice variants lacking the LBD (e.g. AR-V7). Inhibition of the N-terminal domain (NTD) of the AR can inhibit transcriptional activity even in the presence of LBD-driven resistance. A Phase I clinical trial of the first-generation AR NTD inhibitor, EPI-506, demonstrated minor PSA declines in anti-androgen refractory mCRPC patients, revealing the need for more potent and metabolically stable NTD inhibitors. EPI-7386 represents a second generation of NTD inhibitors (Anitens) that are more active and more metabolically stable than EPI-506. Methods: Chemical structure activity relationships were explored to increase molecule potency while maintaining target specificity using a wide variety of CRPC models in vivo and in vitro. The stability and selectivity of the molecules were characterized with a panel of selective screening and functional assays. The mechanism of action and pathway engagement markers were followed by qPCR and RNAseq. Results: EPI-7386 was chosen as the IND candidate. The molecule demonstrated a 20-fold improvement in AR-driven cellular potency compared to EPI-002, while being highly stable in human and animal hepatocytes. In vitro proliferation assays demonstrated on-target activity across a panel of prostate cancer cell lines, with activity demonstrated in AR-V7-driven cellular models. The antiproliferative effect correlated with the inhibitory effect on AR driven genes, including specifically AR-V7 driven genes such as UBE2C. EPI-7386 controlled tumor growth and induced tumor regressions in several CRPC xenografts, including AR-V7-driven 22Rv1 and enzalutamide resistant LNCaP95 models, as well as the VCaP model. Importantly, the combination of enzalutamide with EPI-7386 demonstrated a more robust and consistent PSA and antitumor response in the VCaP model. EPI-7386 was well tolerated in animal models, and therapeutic levels could be achieved without displaying signs of toxicity in a 14-day rat study. Pharmacodynamic markers will be presented, in addition to their incorporation in the future clinical plan. Conclusions: The next generation aniten compound EPI-7386 is more active and metabolically stable than EPI-506. It shows on-target activity in AR full length and AR-V7 driven models, and demonstrates a high therapeutic index in preclinical models. As a single agent, EPI-7386 may overcome anti-androgen clinical resistance in advanced mCRPC as well as potentially in combination therapy with anti-androgens in earlier stages of the disease. The clinical strategy supporting the development of this Aniten N-terminal domain inhibitor of AR will be discussed. Citation Format: Ronan Le Moigne, C. Adriana Banuelos, Nasrin R Mawji, Teresa Tam, Jun Wang, Kunzhong Jian, Raymond J Andersen, Alessandra Cesano, Marianne D Sadar, Han-Jie Zhou, Peter Virsik. Treatment of castrated resistant prostate cancer with EPI-7386, a second generation N-terminal domain androgen receptor inhibitor [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; 2019 Oct 26-30; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2019;18(12 Suppl):Abstract nr B117. doi:10.1158/1535-7163.TARG-19-B117

Ronan Le Moigne; C. Adriana Banuelos; Nasrin R Mawji; Teresa Tam; Jun Wang; Kunzhong Jian; Raymond J Andersen; Alessandra Cesano; Marianne D Sadar; Han-Jie Zhou; Peter Virsik. Abstract B117: Treatment of castrated resistant prostate cancer with EPI-7386, a second generation N-terminal domain androgen receptor inhibitor. Molecular Cancer Therapeutics 2019, 18, B117 -B117.

Ronan Le Moigne, C. Adriana Banuelos, Nasrin R Mawji, Teresa Tam, Jun Wang, Kunzhong Jian, Raymond J Andersen, Alessandra Cesano, Marianne D Sadar, Han-Jie Zhou, Peter Virsik. Abstract B117: Treatment of castrated resistant prostate cancer with EPI-7386, a second generation N-terminal domain androgen receptor inhibitor. Molecular Cancer Therapeutics. 2019; 18 (12_Supplem):B117-B117.

Ronan Le Moigne; C. Adriana Banuelos; Nasrin R Mawji; Teresa Tam; Jun Wang; Kunzhong Jian; Raymond J Andersen; Alessandra Cesano; Marianne D Sadar; Han-Jie Zhou; Peter Virsik. 2019. "Abstract B117: Treatment of castrated resistant prostate cancer with EPI-7386, a second generation N-terminal domain androgen receptor inhibitor." Molecular Cancer Therapeutics 18, no. 12_Supplem: B117-B117.

Bai-Sui Feng; Yong-Jin Wu; Xian-Hai Zeng; Dian Yu; Zhi-Qiang Liu; Cai-Jie Zhou; Peng-Yuan Zheng; Ping-Chang Yang. Bcl2L12 mediates effects of protease-activated receptor-2 on the pathogenesis of Th2-dominated responses of patients with ulcerative colitis. Archives of Biochemistry and Biophysics 2018, 657, 8 -14.

Bai-Sui Feng, Yong-Jin Wu, Xian-Hai Zeng, Dian Yu, Zhi-Qiang Liu, Cai-Jie Zhou, Peng-Yuan Zheng, Ping-Chang Yang. Bcl2L12 mediates effects of protease-activated receptor-2 on the pathogenesis of Th2-dominated responses of patients with ulcerative colitis. Archives of Biochemistry and Biophysics. 2018; 657 ():8-14.

Bai-Sui Feng; Yong-Jin Wu; Xian-Hai Zeng; Dian Yu; Zhi-Qiang Liu; Cai-Jie Zhou; Peng-Yuan Zheng; Ping-Chang Yang. 2018. "Bcl2L12 mediates effects of protease-activated receptor-2 on the pathogenesis of Th2-dominated responses of patients with ulcerative colitis." Archives of Biochemistry and Biophysics 657, no. : 8-14.

Jiatong Han; Donghui Xu; Yanyan Huang; Yuwei Hua; Xin Ding; Zhihao Lin; Jie Zhou; Huan Lin; Ge Chen; Jing Wang; Xiaomin Xu; Jia Liu; Guangyang Liu. Developing fine-tuned MOF membranes for highly efficient separation and adsorption of chemical pollutant in water. Chemical Engineering Journal 2024, 497 .

Jiatong Han, Donghui Xu, Yanyan Huang, Yuwei Hua, Xin Ding, Zhihao Lin, Jie Zhou, Huan Lin, Ge Chen, Jing Wang, Xiaomin Xu, Jia Liu, Guangyang Liu. Developing fine-tuned MOF membranes for highly efficient separation and adsorption of chemical pollutant in water. Chemical Engineering Journal. 2024; 497 ():.

Jiatong Han; Donghui Xu; Yanyan Huang; Yuwei Hua; Xin Ding; Zhihao Lin; Jie Zhou; Huan Lin; Ge Chen; Jing Wang; Xiaomin Xu; Jia Liu; Guangyang Liu. 2024. "Developing fine-tuned MOF membranes for highly efficient separation and adsorption of chemical pollutant in water." Chemical Engineering Journal 497, no. : .

The number of animals born dead, which includes the number of mummified (NM) and stillborn (NS) animals, is the most important trait to directly quantify the reproductive loss in domestic pigs. In this study, 282 Landrace sows and 250 Large White sows were genotyped by sequencing (GBS). A total of 816 and 1068 litter records for NM and NS were collected from them. A genome-wide association study (GWAS) was conducted to reveal the genetic difference between NM and NS. A total of 248 and 10 genome-wide significant SNPs were detected for NM and NS across numerous parities in Landrace pigs. The corresponding numbers for Large White pigs were 175 and 6, respectively. All of the detected SNPs were parity specific for both NM and NS in two breeds. Based on significant SNPs, in total 242 (146 for Landrace pig, 96 for Large White pig) and 10 significant chromosome regions (8 for Landrace pigs, 2 for Large White pigs) were found for NM and NS, respectively. Among them, 237 (142 for Landrace pig, 95 for Large White pig) and 8 significant chromosome regions (6 for Landrace pigs, 2 for Large White pigs) for NM and NS were not reported in previous studies. A list of candidate genes at the identified loci was proposed, including HMGB1, SOX5, KCNJ8, ABCC9 and YY1 for NM, ASTN1 for NS. This is the first time when GBS data was used to identify genetic regions affecting NM and NS in Landrace and Large White pigs. Many identified informative SNPs and candidate genes advance our understanding of the genetic architecture of NM and NS in pigs. However, further studies are needed to validate using larger populations with more breeds.

Pingxian Wu; Kai Wang; Jie Zhou; Qiang Yang; Xidi Yang; Anan Jiang; Yanzhi Jiang; Mingzhou Li; Li Zhu; Lin Bai; Xuewei Li; Guoqing Tang. A genome wide association study for the number of animals born dead in domestic pigs. BMC Genetics 2019, 20, 4 .

Pingxian Wu, Kai Wang, Jie Zhou, Qiang Yang, Xidi Yang, Anan Jiang, Yanzhi Jiang, Mingzhou Li, Li Zhu, Lin Bai, Xuewei Li, Guoqing Tang. A genome wide association study for the number of animals born dead in domestic pigs. BMC Genetics. 2019; 20 (1):4.

Pingxian Wu; Kai Wang; Jie Zhou; Qiang Yang; Xidi Yang; Anan Jiang; Yanzhi Jiang; Mingzhou Li; Li Zhu; Lin Bai; Xuewei Li; Guoqing Tang. 2019. "A genome wide association study for the number of animals born dead in domestic pigs." BMC Genetics 20, no. 1: 4.

Ming-Xu Wang; Long-Ni Liang; Weast Siu Siu; Dan Fan; Hao-Ran Sun; Hui-Hui Zhao; Guang-Jie Zhou; Wen-Jun Wu. Loss accounting of environmental pollution within Pearl River Delta region, South China. 2019, 249, 676 -685.

Ming-Xu Wang, Long-Ni Liang, Weast Siu Siu, Dan Fan, Hao-Ran Sun, Hui-Hui Zhao, Guang-Jie Zhou, Wen-Jun Wu. Loss accounting of environmental pollution within Pearl River Delta region, South China. . 2019; 249 ():676-685.

Ming-Xu Wang; Long-Ni Liang; Weast Siu Siu; Dan Fan; Hao-Ran Sun; Hui-Hui Zhao; Guang-Jie Zhou; Wen-Jun Wu. 2019. "Loss accounting of environmental pollution within Pearl River Delta region, South China." 249, no. : 676-685.

Yi Fang; Xin Zhang; Jie Zhou; Jingwen Guo; Xun Huang. Acoustic metaporous layer with composite structures for perfect and quasi-omnidirectional sound absorption. Composite Structures 2019, 223 .

Yi Fang, Xin Zhang, Jie Zhou, Jingwen Guo, Xun Huang. Acoustic metaporous layer with composite structures for perfect and quasi-omnidirectional sound absorption. Composite Structures. 2019; 223 ():.

Yi Fang; Xin Zhang; Jie Zhou; Jingwen Guo; Xun Huang. 2019. "Acoustic metaporous layer with composite structures for perfect and quasi-omnidirectional sound absorption." Composite Structures 223, no. : .

The E-skin is a key component for use ranging from wearable electronic devices to human-machine interactions. Previous studies proposed that a parallel plate capacitor with a PDMS dielectric layer of micro-pyramids showed good pressure sensing abilities, but overlooked effects of the flat PDMS layers at the top and bottom of these microstructures induced by fabrication processes of soft imprinting. In this work, these effects were firstly studied by the finite element method. The second-order Ogden model was employed to describe the nonlinear mechanical behaviors of the PDMS. We found that the E-skin sensing unit showed increased sensitivity but decreased linearity with the increase of the pyramid height ratio. 48% pyramid height ratio was therefore applied for experimental studies for compromising the performances. We demonstrated an easy accessible and cost-effective method of fabricating the sensing unit, by using a commercial precision abrasive sheet as the mold of the microstructured dielectric film. We introduced a prefilling step to control the pyramid height ratio. Experimental results proved that the sensitivity, pressure resolution and time response of the sensor unit with pyramid height ratio of 45% were better than that with 0. We further demonstrated its applications as a wearable health monitoring device and an approaching and touch sensing unit for a 3D-printed soft hand, showing its great potentials in continuous health monitoring sensors, as well as large area human-machine interactions.

Bin He; Zhe Yan; Yanmin Zhou; Jie Zhou; Qigang Wang; Zhipeng Wang. FEM and experimental studies of flexible pressure sensors with micro-structured dielectric layers. Journal of Micromechanics and Microengineering 2018, 28, 105001 .

Bin He, Zhe Yan, Yanmin Zhou, Jie Zhou, Qigang Wang, Zhipeng Wang. FEM and experimental studies of flexible pressure sensors with micro-structured dielectric layers. Journal of Micromechanics and Microengineering. 2018; 28 (10):105001.

Bin He; Zhe Yan; Yanmin Zhou; Jie Zhou; Qigang Wang; Zhipeng Wang. 2018. "FEM and experimental studies of flexible pressure sensors with micro-structured dielectric layers." Journal of Micromechanics and Microengineering 28, no. 10: 105001.

Jingyi Zhu; Jie Zhou; Zhaozhong Yang; Xinjie Xie; Xiaogang Li; Puzhi Li. Preparation and performance evaluation of microencapsulated acid used for gel breaking of fracturing fluid in low-temperature reservoirs. Journal of Molecular Liquids 2024, 403 .

Jingyi Zhu, Jie Zhou, Zhaozhong Yang, Xinjie Xie, Xiaogang Li, Puzhi Li. Preparation and performance evaluation of microencapsulated acid used for gel breaking of fracturing fluid in low-temperature reservoirs. Journal of Molecular Liquids. 2024; 403 ():.

Jingyi Zhu; Jie Zhou; Zhaozhong Yang; Xinjie Xie; Xiaogang Li; Puzhi Li. 2024. "Preparation and performance evaluation of microencapsulated acid used for gel breaking of fracturing fluid in low-temperature reservoirs." Journal of Molecular Liquids 403, no. : .